sgrna cloning vector (Addgene inc)
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Addgene inc
sgrna cloning vector
Sgrna Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+sgrna+cloning+vector/CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning+vector+(Plasmid+%23159281)/pmc12097578-49-1-9
Average 93 stars, based on 3 article reviews
Sgrna Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+sgrna+cloning+vector/CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning+vector+(Plasmid+%23159281)/pmc12097578-49-1-9
Average 93 stars, based on 3 article reviews
sgrna cloning vector - by Bioz Stars,
2026-09
93/100 stars
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Cloning:Article Title: A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease Article Snippet: The CMV promoter-driven Cas9 plasmid was purchased from Addgene (#41815). .. The Article Title: Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators Article Snippet: To construct pCMV-dCAS9-VP64 vector, hCAS9 vector (#41815, Addgene, a gift from George Church) was mutated (D10A + H840A) and named dCAS9, and VP64 activation domain module was fused with the dCAS9 gene as previously described . .. Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Article Title: SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury Article Snippet: To generate TLR2 homozygous knockout LX-2 cells, two guide RNAs targeting exon 3 of TLR2 gene was designed by using online available software ( https://www.atum.bio/eCommerce/cas9/input ). .. The two sgRNAs were ligated to the Article Title: Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Article Snippet: .. Next, two sgRNAs were synthesized and ligated to Article Title: Efficient base editing for multiple genes and loci in pigs using base editors Article Snippet: .. The obtained vectors were named as pCS2-BE3 and pCDNA3.1-BE3. pCMV-hA3A-BE3 vector (#113410) and Plasmid Preparation:Article Title: A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease Article Snippet: The CMV promoter-driven Cas9 plasmid was purchased from Addgene (#41815). .. The Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Article Title: SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury Article Snippet: To generate TLR2 homozygous knockout LX-2 cells, two guide RNAs targeting exon 3 of TLR2 gene was designed by using online available software ( https://www.atum.bio/eCommerce/cas9/input ). .. The two sgRNAs were ligated to the Article Title: Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Article Snippet: .. Next, two sgRNAs were synthesized and ligated to Construct:Article Title: A huntingtin knock-in pig model recapitulates features of selective neurodegeneration in Huntington’s disease Article Snippet: The CMV promoter-driven Cas9 plasmid was purchased from Addgene (#41815). .. The Article Title: Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators Article Snippet: To construct pCMV-dCAS9-VP64 vector, hCAS9 vector (#41815, Addgene, a gift from George Church) was mutated (D10A + H840A) and named dCAS9, and VP64 activation domain module was fused with the dCAS9 gene as previously described . .. Article Title: Efficient base editing for multiple genes and loci in pigs using base editors Article Snippet: .. The obtained vectors were named as pCS2-BE3 and pCDNA3.1-BE3. pCMV-hA3A-BE3 vector (#113410) and Synthesized:Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Article Title: Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Article Snippet: .. Next, two sgRNAs were synthesized and ligated to Polymerase Chain Reaction:Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Clone Assay:Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Expressing:Article Title: Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Article Snippet: 1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, China; 2CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; 3School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China; 4School of Life Sciences, University of Science and Technology of China, Hefei, China; 5Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China; 6Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou, China Article Title: SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury Article Snippet: To generate TLR2 homozygous knockout LX-2 cells, two guide RNAs targeting exon 3 of TLR2 gene was designed by using online available software ( https://www.atum.bio/eCommerce/cas9/input ). .. The two sgRNAs were ligated to the Article Title: Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Article Snippet: .. Next, two sgRNAs were synthesized and ligated to |
